Part:BBa_K4429011

NeoFv C10 VH-CycY12H-VL-Lin

NeoFvs are engineered antibody fragments. An scFv is defined as the variable domains of a full length antibody connected via a flexible linker. A NeoFv is a name given by iGEM 2022 team IISER-Pune2_India to scFvs with a small peptide extension that binds to the human neonatal Fc receptor (or FcRn receptor). Such scFvs with an FcRn binding peptide have recently been described in literature[1] with potential to overcome the size problem of scFvs, and extend their half life of circulation in the human body.

NeoFv C10 VH-CycY12H-VL-Lin is one such NeoFv derived from the C10 antibody targeting the envelope-dimer epitope of the Dengue virus. A NeoFv derived from C10 can ideally be a therapy for Dengue fever. -

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• 1]Short FcRn-Binding Peptides Enable Salvage and Transcytosis of scFv Antibody Fragments

Vince W. Kelly and Shannon J. Sirk ACS Chemical Biology 2022 17 (2), 404-413 DOI: 10.1021/acschembio.1c00862